Journal: PLoS ONE

Article Title: Neutrophils remain detrimentally active in hydroxyurea-treated patients with sickle cell disease

doi: 10.1371/journal.pone.0226583

Figure Lengend Snippet: (A) Purified and rested neutrophils from healthy donors (H) and SCD patients in steady state (SS) were left untreated in RPMI as control or treated with 20 μM hemin. Median Fluorescence Intensity of F-Actin (upper panel) and CD63 (lower panel) in F-Actin/CD63 double positive neutrophils with normal multi-lobulated nuclei is shown. (Healthy N = 7; SCD N = 6). (B) Z-stack stills of SCD neutrophils left in RPMI for 30 minutes showing neutrophils with roughed cell membranes (yellow arrows, upper panel). Staining for DNA (blue), F-Actin (green) and CD63 (red) is presented (lower panel). (C) MPO release was assayed by ELISA following 30 minutes incubation with or without the hemin stimulus (H (Healthy) N = 4; SS (SCD) N = 4). Degranulation response was assayed in terms of MPO ng/ml plasma (upper panel), or fold increase over healthy neutrophils (lower panel). Data presented as dot plots±S.D., significance calculated with an unpaired Mann-Whitney test.

Article Snippet: The supernatants were then assayed with a Human Myeloperoxidase Quantikine ELISA Kit from R&D Systems according to the manufacturer’s instruction.

Techniques: Purification, Control, Fluorescence, Staining, Enzyme-linked Immunosorbent Assay, Incubation, Clinical Proteomics, MANN-WHITNEY

Journal: Dose-Response

Article Title: Barbaloin Treatment Contributes to the Rebalance of Glucose and Lipid Homeostasis of Gestational Diabetes Mellitus Mice

doi: 10.1177/1559325820984910

Figure Lengend Snippet: Barbaloin decreased the ROS levels in gestational diabetes mellitus mice. Malondialdehyde (MDA) (A), superoxide dismutase (SOD) (B), glutathione peroxidase (GPx) (C), glutathione (GSH) (D) in liver tissue were measured by ELISA on GD 20 among different groups. Data are presented as mean ± SD. n = 8 for each group. **p < 0.01, ***p < 0.001 compared to wild type group, & p < 0.05, && p < 0.01 compared to GDM mice.

Article Snippet: The levels of malondialdehyde (MDA) (ab118970, Abcam, USA), superoxide dismutase (SOD) (ab65354, Abcam, USA), glutathione peroxidase (GPx) (ab102530, Abcam, USA), and glutathione (GSH) (NBP2-68015, NOVUS Biologicals, Littleton, CO, USA) in liver tissue on GD 20 were measured by colorimetric kit according to manufactory’s instruction.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Antioxidants

Article Title: Vitamin D Mitigates Hyperglycemia-Induced Cognition Decline in Danio rerio (Zebrafish) through the Activation of Antioxidant Mechanisms

doi: 10.3390/antiox11112114

Figure Lengend Snippet: Hyperglycemia-induced oxidative stress markers were modified by vitamin-D and sulforaphane. ( A , B ) Vitamin D and SFN could mitigate the hyperglycemia-induced changes in the reduced-glutathione (GSH) and malondialdehyde (MDA) levels in zebrafish. Hyperglycemia has decreased the endogenous glutathione level in zebrafish compared with normal control ones. Treatment with vitamin D and SFN moderately increased the GSH level in hyperglycemia zebrafish ( A ). The oxidative stress marker MDA (a product of lipid peroxidation) has increased in the hyperglycemia zebrafish. Elevated MDA level was decreased by the treatment with vitamin D and SFN ( B ). The “***” & “**” represents a significant difference compared with that of normal control group with p < 0.0001 & <0.05. “$$$” & “$$” Represents a significant difference compared with that of the disease control group with p < 0.0001 & <0.05. ‘ns’ represents a non-significance. ( C – E ) Treatment with vitamin D and SFN modulated the activity of glutathione peroxidase (GPX), superoxide dismutase (SOD) and NADPH quinone oxidoreductase 1 (NQO1) in zebrafish. Hyperglycemic state has reduced the GPX while increasing SOD and NQO1 activity in zebrafish ( C – E ). Treatment with vitamin D and SFN has increased the GPX level while decreasing diabetes-induced SOD and NQO1 activity. The data represents mean ± SEM. The results were analyzed by one-way ANOVA, followed by Tukey’s post-hoc test, used for multiple comparisons. The “***”, “**” & ‘’*’’represents a significant difference compared with that of the normal control group with p < 0.0001, <0.001 & <0.05. “$$$”, “$$” & ‘’$’’ Represents a significant difference compared with that of the disease control group with p < 0.0001, <0.001 & <0.05. ‘ns’ represents non-significance.

Article Snippet: Glutathione Peroxidase Activity (GPx) Assay: Glutathione peroxidase activity was determined in the whole brain homogenates by using commercially available high throughput glutathione peroxidase assay kit from Trevigen (catalogue no:7512-100-K).

Techniques: Modification, Control, Marker, Activity Assay

Journal: Bioengineered

Article Title: Effect of α-tocopherol in alleviating the lipopolysaccharide-induced acute lung injury via inhibiting nuclear factor kappa-B signaling pathways

doi: 10.1080/21655979.2022.2031399

Figure Lengend Snippet: α-TOH reduces LPS-induced oxidative stress in lung tissue of mice. (a) IHC staining results of SOD1 and SOD2 in mice lung (400×); (b) Western blot results of SOD1 and SOD2; (c–f) The concentration of MPO, MDA, SOD, and GSH-Px in mice serum. (‘*’ means p < 0.05 vs. control group and ‘#’ means p < 0.05 vs LPS group)

Article Snippet: After collecting the mouse serum, the manufacturer’s instructions given on the MPO kit (R&D Systems, Emeryville, CA, USA), MDA kit (R&D Systems, Emeryville, CA, USA), SOD kit (R&D Systems, Emeryville, CA, USA) and GSH-Px kit (R&D Systems, Emeryville, CA, USA) were followed to detect the oxidative stress level in the mouse.

Techniques: Immunohistochemistry, Western Blot, Concentration Assay, Control